EFFECT OF EQUINE XENOGRAFT ON GENE EXPRESSION OF DENTAL PULP STEM CELLS IN VITRO

¹ Dental School, Albanian University, Tirana, Albania
² Dept of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

Correspondence to:

Annals of Stomatology 2023 January-April; 3(1): 30-37
https://doi.org/10.69129/stomatol/2023v3iss1_2

Received: 10 March 2023 Accepted: 5 April 2023

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Abstract

Bone grafting is a surgical procedure used to repair complex bone deficit. The damaged bone is replaced with a graft that supports bone regeneration and healing of bone defects. This technique is vital in orthopedics, dentistry, and trauma surgery, addressing the conditions that traditional methods cannot effectively treat. Bone grafts can originate from various sources, including the patient’s body, donors, or synthetic materials, each with distinct advantages and challenges. In this study, we cultured dental pulp stem cells to determine whether equine xenograft (EQX) bones can promote osteoblast differentiation. The gene expression of a panel of 15 differentiation markers was analyzed at two-time points. After 24 h of treatment, FOSL1, SPP1, and MMP14 expression were upregulated. After four days, increased expression of SP7, COL1A1, and COL4A1 was observed. This study indicates that EQX could be considered a material that favors bone regeneration by promoting osteoblast differentiation.

Keywords: bone, expression, graft, osteoblasts, stem