GENE EXPRESSION OF STEM CELLS TREATED WITH MARINE-DERIVED POROUS CARBONATED ALGA IN VITRO

¹ Dental School, Albanian University, Tirana, Albania
² Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

Correspondence to:

Annals of Stomatology 2022 January-April; 2(1): 17-22
https://doi.org/10.69129/stomatol/2022v2iss1_4

Received: 24 January 2022 Accepted: 26 February 2022

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Abstract

Marine-derived porous carbonated red algae (MDPCRA) is a bone substitute for bone regeneration in dentistry. It is manufactured through a controlled process, resulting in a porous scaffold with interconnected pores and a high surface area conducive to cell attachment, proliferation, and tissue ingrowth. MDPCRA exhibits excellent biocompatibility, osteoconductivity, and resorbability, making it an ideal substrate for bone regeneration and remodeling. Dental follicle stem cells (DFSCs) are a population of mesenchymal stem cells found within the dental follicle surrounding developing teeth. DFSCs exhibit self-renewal capacity and multipotent differentiation potential, allowing them to differentiate into various cell types, including osteoblasts, cementoblasts, adipocytes, and periodontal ligament fibroblasts. To verify how MDPCRA stimulates bone regeneration, we treated dental DFSCs with MDPCRA to obtain information regarding the expression of genes related to osteoblast differentiation. In DFSCs, after 24 h of treatment, MMP15 was up-regulated. After 4 days of treatment, MMP15 still increased, and IL6 and SP7 were also upregulated. In conclusion, MDPCRA can stimulate several genes in DFSCs involved in osteoblast differentiation.

Keywords: bone regeneration, dental follicle, gene expression, marine-derived porous carbonated red alga, stem cells