Journal of Orthopedics

Evaluation Study

ANORGANIC BOVINE BONE MATRIX TREATED WITH P15 EFFECTS IN OSTEOBLASTIC STEM CELL DIFFERENTIATION

P. Daliu1 ORCID, B. Zeza2, M. Martinelli3 ORCID

1 Department of Pharmacology, Albanian University, Tirana, Albania
2 Dental School, Albanian University, Tirana, Albania
3 Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

Correspondence to:

Marcella Martinelli, PhD
Department of Medical and Surgical Sciences,
University of Bologna,
40138 Bologna, Italy
e-mail: marcella.martinelli@unibo.it

Journal of Orthopedics 2023 September-December; 15(3): 110-116


Received: 12 August 2023 Accepted: 10 September 2023

Copyright © by LAB srl 2023 ISSN 1973-6401
This publication and/or article is for individual use only and may not be further reproduced without written permission from the copyright holder. Unauthorized reproduction may result in financial and other penalties. Disclosure: All authors report no conflicts of interest relevant to this article.

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Abstract

Molecular interaction between stem cells and grafting biomaterial is a key factor for the success of regenerative surgery. The covalent attachment of P15, a synthetic peptide mimicking the cell-binding site of collagen type I, to biomaterials could favor integrins binding and activation of multiple cell signaling pathways. In this investigation, we studied the effect of P15-coated anorganic bone matrix on bone marrow stem cells cultured in vitro. The expression level of a panel of differentiation markers was monitored to verify cell differentiation towards the osteoblast lineage. We observed some signs of osteoblast differentiation, such as the over-expression of osterix and under-expression of endoglin. However, most typical markers, osteonectin, osteopontin, osteocalcin, and alkaline phosphatase, were strongly under-expressed.

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