ANORGANIC BOVINE BONE MATRIX TREATED WITH P15 EFFECTS IN OSTEOBLASTIC STEM CELL DIFFERENTIATION

P. Daliu¹ , B. Zeza², M. Martinelli³

¹ Department of Pharmacology, Albanian University, Tirana, Albania
² Dental School, Albanian University, Tirana, Albania
³ Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

Correspondence to:

Journal of Orthopedics 2023 September-December; 15(3): 110-116
https://doi.org/10.69149/orthopedics/2023v15iss3_7

Received: 12 August 2023 Accepted: 10 September 2023

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Abstract

Molecular interaction between stem cells and grafting biomaterial is a key factor for the success of regenerative surgery. The covalent attachment of P15, a synthetic peptide mimicking the cell-binding site of collagen type I, to biomaterials could favor integrins binding and activation of multiple cell signaling pathways. In this investigation, we studied the effect of P15-coated anorganic bone matrix on bone marrow stem cells cultured in vitro. The expression level of a panel of differentiation markers was monitored to verify cell differentiation towards the osteoblast lineage. We observed some signs of osteoblast differentiation, such as the over-expression of osterix and under-expression of endoglin. However, most typical markers, osteonectin, osteopontin, osteocalcin, and alkaline phosphatase, were strongly under-expressed.

Keywords: bone marrow stem cells, bone regeneration, gene expression, p15